Abstract
The endogenous avian leukosis virus genome of uninfected virus-producing(V +) chicken embryo fibroblasts appears to be inefficiently transcribed and is noninfectious in transfection assays of high molecular weight DNA. We previously proposed that the endogenous virus genome was linked to a cis-acting control element which inhibited its transcription and, consequently, interfered with transfection by endogenous viral DNA. We now report that DNA of uninfected V + cells is infectious at a low level when sheared to the approximate size of the virus genome. These results suggest that the endogenous virus genome of uninfected V + chicken cells can be converted to an infectious form by separating it from adjacent cellular DNA sequences, as predicted by the cis-acting control element model.
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