Abstract
Genetic linkage analysis was performed using two segregating populations of citrus. One population arose from an intergeneric backcross of Citrus grandis (L.) Osb. cv 'Thong Dee' and Poncirus trifoliata (L.) Raf. cv 'Pomeroy', using the former as the recurrent (female) parent. The other population came from an interspecific backcross of C. reticulata Blanco cv 'Clementine' and C. x paradisi Macf. cv 'Duncan', using the former as the recurrent (male) parent. A total of 11 isozyme and 58 restriction fragment length polymorphisms were found to segregate in a monogenic fashion in one or both populations. Linkage analysis revealed that 62 of the loci examined mapped to 11 linkage groups, while 7 loci segregated independently from all other markers. Gene order was highly conserved between the maps generated from the two divergent segregating populations. Possible applications of the use of such maps in tree fruit breeding are discussed.
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