Linkage of Metabolic Disorders, Endothelial Dysfunction and NOS3 (rs2070744) and GNB3 (rs5443) Genes Polymorphisms in Hypertensive Patients

Abstract

The vascular endothelium is one of the earliest damage-targeted hypertensive-mediated organs. The study aims to analyze the link of metabolic disorders with endothelial dysfunction (ED) and NOS3 (rs2070744) and GNB3 (rs5443) genes polymorphism in essential arterial hypertension (EAH). One hundred EAH patients (48 – healthy control) participated in the case-control study. Creatinine, glucose, triglycerides, total cholesterol (TC), low/high-density lipoproteins values (LDL-C, HDL-C), Atherogenicity Index (AI), Soluble Vascular Cell Adhesion Molecule, total NO metabolites (NO2-/NO3-) were studied. GNB3 (rs5443) and NOS3 (rs2070744) genotyping performed by TaqMan probes (CFX96™Real-Time PCR). Moderate-severe ED in EAH patients associated with higher blood pressure – by 6.90% and 4.69% (Р<0.05), creatinine blood increase – by 10.08% (Р=0.037), profound dysmetabolic changes but only in men: hyperglycemia by 46.46% (Р=0.004), hypercholesterolemia and lower HDL-C content by 15.79% (Р=0.024), AI elevation by 33.93% (Р=0.029). ТТ-genotype of GNB3 gene patients has a higher TC blood content by 13.97% (Р=0.035). The one-way ANOVA analysis did not confirm the linkage of the NOS3 (rs2070744) and GNB3 (rs5443) genes polymorphic site with metabolic disorders. Meanwhile, the risk of obesity increases in EAH patients with C-allele of NOS3 gene and T-allele of GNB3 gene almost 6 and 10 times [OR95%CI:2.11-14.82; P<0.001 and OR 95%CI:2.25-45.44; P<0.001], respectively.