Abstract
Clone QEI (QEI-40, D10752), which contains a full-length cDNA encoding the starch branching enzyme I, or Q-enzyme I, was isolated from a cDNA library of developing rice endosperm in λgt11 (Nakamura and Yamanouchi 1992). Based on the sequence of this clone, we synthesized four oligonucleotides which were used as primers for the polymerase chain reaction amplification (Table 1). Using these synthesized primers and λgt11 primers (Takara), three kinds of PCR products were prepared as probes: namely, a full-length cDNA (QEI-40-1), the open reading frame region (QEI-40-2), and the 3' untranslated region which is specific for rice QEI (QEI-40-3). These three probes were employed for RFLP analysis and linkage mapping
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