Linkage Analysis of Nine Isozyme Genes on the Conventional Linkage Map in Rice.

Abstract

The locations of nine isozyme genes on conventional linkage maps were studied in rice (Oryza sativa L.). Two isozyme genes on chromosome 12 showed a linkage with the gene for bonsaito dwarf (d-33) which is a marker on the same chromosome. Based on the data, the gene order was estimatecl as Acp-1-Pox-2-d-33. Pgd-1 was found to be linked to la, v-4 and Adh-1 on chromosome 11. It was possible to confirm that the order of these genes was la, v-4-Pgd-1-Adh-1 based on the linkage relations. From the co-segregation of Pgd-2 and other isozyme genes, linkage relations with Est-2 and Pgi-2 were detected on chromosome 6. Newly acquired gene (Gdh-1) was linked to Pgi-1. The genes were assigned to chrorno-some 3, because Pgi-1 showed linkage with two morphological markers, chl-1 and v-1 which are located on chromosome 3. Based on the linkage data, it was confirmed that the order of these genes was chl-1-Pgi-1-v-1. In total, new linkage relations of nine isozyme genes were determined. These and published data were used in linkage maps constructed with 11 isozyme *crenes and six morphological markers on conventional linkage maps. The genes were distrlbuted to four out of twelve chromosomes in rice.