Link between PON 1 gene mutation and recurrent miscarriage among women exposed to pesticides in North India is insignificant

Akancha Pandey

doi:10.6026/97320630016779

Abstract

Recurrent miscarriage is a loss of disconcerting disorder characterized by RPL (recurrent pregnancy loss) of pregnancy, affecting around 1-2% of couples trying to conceive. Exposure to pesticide affects spontaneous abortion, and infertility in women. Placental oxidative stress is often linked to miscarriage. Therefore, it is of interest to link PON1 (Q1922R) polymorphism with recurrent pregnancy loss. We selected 200 subjects in which 100 patients with RPL having consecutive 2 or more miscarriages and 100 healthy controls from the northern India for this study. Blood samples were collected for DNA isolation and further assessment. Genotyping of the Q1922R polymorphism was completed using the RFLP markers. The digested PCR product size was 99 bp (control). The heterozygous fragments were found to be 66 and 33 bp homozygous mutants. It was observed that allele frequency homozygous (TT) was more prevalent among control than the case groups among the healthy north-Indian population. However, heterozygous group (Tt) was more in cases compared to control groups as well as homozygous mutant was observed high in control in than case (CI-0.3 to 1.3).

Highlights

Recurrent miscarriage is a loss of disconcerting disorder characterized by RPL of pregnancy, affecting around 12% of couples trying to conceive
Various studies has been identified in association between the polymorphism and risk of different diseases such as in previous reports it was suggested that there is a high variability in PON1 enzymes related to various problems including recurrent pregnancy loss [16, 13, 17], female infertility [18], Coronary heart disease [19, 20, 21, 22, 23], Cancer [24, 25], Interlekin [26], hypercholesterolemia [27], Osteonecrosis [28], Metabolic Syndrome [29], atherosclerosis [30]
This study evaluates the interaction between exposure to pesticides and PON1 gene polymorphism in recurrent miscarriages in northern Indian females

Summary

Introduction

Recurrent miscarriage is a loss of disconcerting disorder characterized by RPL (recurrent pregnancy loss) of pregnancy, affecting around 12% of couples trying to conceive. It is defined as the loss of pregnancy before the fetus reaches the viability, which is from the time of conception until 24 weeks of gestation period [4] It can be caused by multiple factors such as hormonal imbalances, nutritional deficiencies, physiological trauma, menstrual disorders, hematologic disorders, chromosomal aberration, endocrinological disorders, uterine fibroid, uterine pathology, Parental chromosomal anomalies, Heritable thrombophilia, and uterine abnormalities [5, 6, 7, 8, 9] in which exposure of pesticides is one of the most common causes of recurrent miscarriages. It is of interest to link PON1 (Q1922R) polymorphism with recurrent pregnancy loss

Methods

Results

Conclusion