Abstract
Emamectin Benzoate was registered recently in Brazil to control Chrysodeixis includens (Walker, [1858]) (Lepidoptera: Noctuidae) in soybean. We studied the baseline susceptibility of Mato Grosso populations of C. includes to support Insect Resistance Management programs by standard feeding assays on insecticide-surface of artificial diet. Variation in susceptibility between populations was low. The LC50 values from 11 populations varied between 0.015 a 0.065 µg i.a. cm-2. Based on our results, we propose the discriminant concentration of 2.867 µg i.a. cm-2 of emamectin benzoate to monitor insecticide resistance in C. includes.
Highlights
We studied the baseline susceptibility of Mato Grosso populations of C. includes to support Insect Resistance Management programs by standard feeding assays on insecticide-surface of artificial diet
We propose the discriminant concentration of 2.867 μg i.a. cm-2 of emamectin benzoate to monitor insecticide resistance in C. includes
Casos de resistência de C. includens a inseticidas são documentados na literatura aos ingredientes ativos acetato, BHC, DDT, cipermetrina, deltametrina, permetrina, teflutrina, fenvalerato, metomil, paratiom metílico, tiodicarbe e flubendiamida (Owen 2013, IRAC 2019) e baixa suscetibilidade a lambda-cialotrina, teflubenzuron e novaluron (Stacke et al 2019)
Summary
As lagartas foram mantidas em dieta artificial até atingirem a fase de pupa (Parra 2001). Uma lagarta em 3o ínstar foi transferida para cada célula com o auxílio de um pincel e as placas foram mantidas em câmara climatizada (24±2 oC, umidade relativa de 70±10% e de 14 horas de fotofase) por 24 horas. Todas as populações de C. includens coletadas em campo apresentaram elevada suscetibilidade ao benzoato de emamectina, inclusive com valores de CL50 inferiores a população de laboratório. Entretanto, a razão de resistência entre as populações testadas, foram inferiores a 2 para a CL50 e 7 para a Cl99, indicando que as populações de C. includens avaliadas podem ser consideradas como suscetíveis a benzoato de emamectina (Tab. 2). Resposta de concentração-mortalidade (CL; μg i.a. cm-2) de C. includens exposta a benzoato de emamectina
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