Linear solvation energy relationships in the determination of phospholipids by supercritical fluid chromatography

Abstract

The separation of phospholipids was studied using supercritical fluid chromatography. The 2-ethylpyridine (2-EP), hybrid fluoro-phenyl (FP), C18, 2-picolylamine (2-PIC), 1-aminoanthracene (1-AA), DIOL and ethylene bridged hybrid (BEH) were used as stationary phases. Methanol with 0.1% formic acid was used as a co-solvent. For the first time, the linear solvation energy relationships (LSER) were used to evaluate the retention mechanism of phospholipid by SFC. Based on Abrahamʼs equation, the stationary phases were classified according to their interaction properties. The retention mechanism of phospholipid at SFC is based on different interactions depend on columns type. Moreover, structural descriptors of the target compounds were used to describe their chromatographic retention behavior under the optimized SFC conditions.