Abstract

Abstract: The effect of Lp on growth of internodes of Nitellopsis obtusa (Desv. In Lois) J. Gr. was studied, with treatments of HgCl2 and antibodies against aquaporins (anti‐RD28) on the plasmalemma, using laser scanning confocal microscopy, and simultaneous monitoring of cell growth, turgor, Em and Lp using a turgor/membrane potential probe (T‐EP probe). Laser scanning confocal immunofluorescent microscopy indicated the immunoreaction of anti‐RD28 on the plasma membrane. Experiments showed that, following treatment with 25 μM HgCl2, cell growth was entirely and Lp largely inhibited, and no growth recovery was seen even when Lp was completely reversed by replacement of HgCl2 with β‐mercaptoethanol (ME). Anti‐RD28 inhibited both Lp and growth of internodes of N. obtusa by about 80 %, and the possible influences on cell growth of changes in turgor, respiration and pH in cell walls, being excluded during treatment. It was confirmed experimentally that enlargement growth of internodes of N. obtusa was linearly related to cell Lp.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call