Linear-programmed thermal degradation methane chemical-ionization mass spectrometry: II. Defined compounds and lipid-containing envelope constituents from Salmonella

Abstract

Studies of temperature-resolved pyrolysis are reported for lipid preparations cell envelope fractions, and whole cells from Salmonella typhimurium TA-100, and lipopolysaccharides from S. abortus equi, S. enteritidis, S. minnesota, and S. typhimurium. Related and model compounds were examined as well; these included the fatty acids: caproic acid, lauric acid, myristic acid, palmitic acid, stearic acid, linoleic acid, oleic acid; phospholipids and related compounds: dl-α-glycerophosphate (disodium salt), l-α-glycerophosphoryl ethanolamine, l-α-glycerophosphoryl choline, dipalmitoyl dl-α-phosphatidyl ethanolamine, palmitoyl l-α-lysophosphatidyl ethanolamine, palmitoyl l-α-lysophosphatidyl choline, lauroyl l-α-lysophosphatidyl choline, stearoyl l-α-lysophosphatidyl choline, oleoyl l-α-lysophosphatidyl choline; and the lipopolysacchoride constituent 3-deoxy- d-manno-octulosonic acid. Results indicate that certain of the ions appearing in the fingerprints of cell wall components of gram-negative bacteria are present in spectra of related compounds.