Linear B-cell epitope mapping of neuraminidases of the 2009 A H1N1 viruses based on immunoinformatics

Abstract

The 2009 A H1N1 viruses have spread throughout the world, as the viral neuraminidase (NA) is a receptor-destroying enzyme, removing sialic acid from carbohydrate chains attached to NA, and releasing the viruses from infected cells. In this study, the NA genes of Guangdong viruses were sequenced, then their B-cell epitopes were predicted, screened and assessed based on immunoinformatics. The antisera were raised in rabbits against five linear synthetic peptides spanning the NA protein of 2009 A H1N1. Five peptides, LR17, SS12, DP9, DS11 and DI14, respectively, are capable of eliciting neutralizing antibodies against 2009 A H1N1 in the in vitro microneutralization assay. DI14 was identified to be particularly potent in eliciting a neutralizing antibody titer comparable to that obtained with a whole virion-immunized serum. Immunization of rabbit with either five peptides triggered a 2009 A H1N1-specific antibody response as high as that obtained with the whole virion as immunogen. Alignment with databases showed that the amino acid residues of five epitope peptides are highly conserved among the NA sequences of 2009 A H1N1 strains isolated from the world. Altogether, these data indicate that LR17, SS12, DP9, DS11 and DI14 represent a promising candidate for an effective synthetic peptide-based vaccine against 2009 A H1N1 viruses.