Abstract
Linear-after-the-exponential (LATE)-PCR describes a novel approach to asymmetric PCR which uses adjusted melting temperatures of the limited primer to increase PCR efficiency. In this proof-of-principle study we show that linear amplification is possible over a wide range of amplification cycles. The curve characteristics of the real time PCR show that the plateau effect of conventional PCR can be avoided by this innovative technique.
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More From: Forensic Science International: Genetics Supplement Series
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