Abstract
The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.
Highlights
The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease
International authorities are deploying another 3.5 million doses of the standard vaccine strain, 17D (6). Both vaccine strains originate from the same parental strain, Asibi, and represent the West African genotype 2
In YFV-endemic regions, it is essential to distinguish between YF-VAAE and wild-type YFV infection (10)
Summary
The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We present 2 highly sensitive real-time reverse transcription PCRs (RT-PCRs) designed to detect and discriminate between YFV vaccine and American wild-type (hereafter referred to as wild-type) strains within 1 hour. We designed 5 different single-target assays using primers capable of simultaneously amplifying vaccine and wild-type YFV strains.
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