Abstract
Brillouin spectroscopy probes the mechanical properties of material by measuring the optical frequency shift induced by photon-phonon scattering interactions. In traditional configurations, Brillouin spectrometers measure only one point of the sample at a time. This results in long acquisition times for mechanical imaging of large areas. In this work, we demonstrate a parallel detection configuration where the Brillouin shift of hundreds of points in a line can be measured simultaneously. In mm-sized samples, this novel configuration effectively shortens the acquisition time of two-dimensional Brillouin imaging from hours to tens of seconds, thus making it a powerful technology for label-free mechanical characterization of tissue and biomaterials.
Highlights
Brillouin spectroscopy allows non-invasive measurement of material mechanical properties by measuring the frequency spectrum of acoustically-induced light scattering within a sample[1]
We used a homebuilt setup of line-scanning Brillouin spectroscopy
The spectral dispersion in the figure is along the vertical axis, while the horizontal axis represents the spatial domain within the sample
Summary
Brillouin spectroscopy allows non-invasive measurement of material mechanical properties by measuring the frequency spectrum of acoustically-induced light scattering within a sample[1]. We devised a novel configuration based on spontaneous Brillouin scattering that allows hundreds of points in a sample to be measured simultaneously using line-scanning parallel detection of scattering spectra A line beam is created into the sample by focusing the incoming laser with a low-NA objective lens With this setup, we reached the equivalent acquisition time of less than 1 ms per sample point, two orders of magnitude shorter than any previous protocols of Brillouin microscopy. A high-resolution two-dimensional Brillouin image can be obtained in a matter of seconds by scanning the sample only in one dimension compared to ~hour required in traditional confocal configurations
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