Abstract
Lincomycin resistant cell lines were screened in monoploid (X = 10 chromosomes) protoplast cultures of Nicotiana plumbaginifolia. Lincomycin is an inhibitor of protein synthesis on plastid ribosomes and normally inhibits greening of cultured cells on RMOP medium. The LR400 line was isolated by its ability to form a green callus on selective medium (RMOP medium containing 1,000 µg ml(-1) lincomycin hydrochloride). Diploid plants regenerated from this line inherited the resistance maternally. The LR400 line is cross-resistant to cleocin (clindamycin), but is sensitive to streptomycin.
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