Abstract
BackgroundNon-small cell lung cancer (NSCLC) is a common malignancy around the globe. Increasing long non-coding RNAs (lncRNAs) have been confirmed to be associated with the progression of cancers, including NSCLC. Long intergenic non-protein coding RNA 1783 (LINC01783) is a novel lncRNA and its regulatory function as competing endogenous RNA (ceRNA) has not been studied in NSCLC.MethodsRT-qPCR measured the expression level of LINC01783 in NSCLC cells. CCK-8, EdU, transwell and wound healing assays were conducted to detect cell proliferation, migration and invasion in NSCLC. The relationship between miR-432-5p and LINC01783 along with delta like 1 (DLL-1) was illustrated by RNA pull down, RIP and luciferase reporter assays.ResultsLINC01783 was found remarkably increased in NSCLC cell lines, and down-regulation of LINC01783 suppressed cell proliferation, migration and invasion. Then, we discovered Notch pathway was related to the progression of NSCLC, and DLL-1 expression was reduced by LINC01783 knockdown. Furthermore, DLL-1 overexpression could counteract the suppressive effects of LINC01783 down-regulation on the growth of NSCLC cells. MiR-432-5p was observed to be the mutual miRNA that could bind with both LINC01783 and DLL-1. Overexpression of miR-432-5p inhibited DLL-1 expression. In the rescue assays, miR-432-5p depletion offset the impacts of LINC01783 knockdown, and then DLL-1 silence recovered the influence of miR-432-5p down-regulation on NSCLC cell growth.ConclusionLINC01783 aggravates NSCLC cell growth by regulating Notch pathway and sponging miR-432-5p, being a potential target in the treatment for NSCLC.
Highlights
Non-small cell lung cancer (NSCLC) is a common malignancy around the globe
LINC01783 is overexpressed and promotes cell proliferation and migration in NSCLC To explore the biological role of LINC01783 in NSCLC cells, Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to measure LINC01783 expression in NSCLC cell lines
It was revealed that LINC01783 was conspicuously overexpressed in NSCLC cell lines (NCI-H460, NCI-H1975, NCI-H1299, and A-549) compared with normal lung epithelial cell line (BEAS-2B) (Fig. 1a)
Summary
Increasing long noncoding RNAs (lncRNAs) have been confirmed to be associated with the progression of cancers, including NSCLC. The statistics showed that 57% lung cancer patients at metastatic stage were with a dramatically low 5-year survival rate of 5% while the survival. Deng et al Cancer Cell Int (2021) 21:234 conventional chemotherapy remain the main treatment methods for lung cancer patients [5]. The long non-coding RNAs (lncRNAs) have been involved in the molecular diagnosis, targeted therapy, and predicting prognosis of lung cancer [6]. Great advancements have been achieved in the diagnosis and treatment, the overall survival rate is still very low and the recurrence possibility is increasing due to metastasis and chemoresistance [7, 8]. It is extremely necessary to find out effective biomarkers for NSCLC treatment
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