LINC01308 accelerates the malignant progression of ovarian cancer by binding to miRNA-506.

Abstract

To detect the expression pattern of LINC01308 in ovarian cancer (OC), and further clarify whether LINC01308 could promote the malignant progression of OC by mediating microRNA-506 (miRNA-506). Relative level of LINC01308 in 28 pairs of OC tissues and paracancerous tissues was determined by quantitative Real-time polymerase chain reaction (qRT-PCR). We analyzed the correlation between LINC01308 level and the prognosis of OC patients. Subsequently, LINC01308 level in OC cell lines was determined as well. By transfection of sh-LINC01308 in 3AO and CAOV3 cell lines, we evaluated the influence of LINC01308 on cellular behaviors of OC through cell counting kit-8 (CCK-8), transwell and wound healing assay. Target downstream of LINC01308 was verified by dual-luciferase reporter gene assay. Finally, the regulatory effect of LINC01308/miRNA-506 on OC cell behaviors was examined through a series of rescue experiments. LINC01308 was highly expressed in OC tissues relative to controls. OC patients with high-level LINC01308 had higher rates of lymph node metastasis and distant metastasis, and lower survival rate compared with those with low level. By transfection of sh-LINC01308 in OC cells, the migratory and invasive abilities were markedly weakened. MiRNA-506 was found to be the target gene of LINC01308, and its level was negatively regulated by LINC01308 in OC tissues. Finally, we found that miRNA-506 knockdown reversed the regulatory effect of LINC01308 on the migratory and invasive abilities of OC cells. LINC01308 is highly expressed in OC and correlated to metastasis and poor prognosis. LINC01308 enhances OC cells to migrate and invade by targeting miRNA-506.