Abstract
BackgroundLong noncoding RNAs (lncRNAs) are related to colorectal cancer (CRC) development. However, the role and mechanism of lncRNA LINC01224 in CRC development are largely unknown.MethodsLINC01224, Yin Yang 1 (YY1), microRNA (miR)-485-5p, and myosins of class VI (MYO6) levels were examined using quantitative reverse transcription polymerase chain reaction and western blotting. Functional analyses were processed through CCK-8, colony formation, flow cytometry, transwell, and xenograft analyses. Dual-luciferase reporter, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation, and pull-down assays were conducted to analyze the binding interaction.ResultsLINC01224 abundance was elevated in CRC tissue samples and cell lines. Elevated LINC01224 might indicate the lower 5-year overall survival in 52 CRC patients. LINC01224 was upregulated via the transcription factor YY1. LINC01224 knockdown restrained CRC cell proliferation, migration, and invasion and increased apoptosis. MiR-485-5p was sponged by LINC01224, and miR-485-5p downregulation relieved the influence of LINC01224 interference on CRC progression. MYO6 was targeted via miR-485-5p and regulated via LINC01224/miR-485-5p axis. MiR-485-5p overexpression suppressed CRC cell proliferation, migration, and invasion and facilitated apoptosis. MYO6 upregulation mitigated the role of miR-485-5p. LINC01224 knockdown decreased xenograft tumor growth.ConclusionYY1-induced LINC01224 regulates CRC development via modulating miR-485-5p/MYO6 axis.
Highlights
Colorectal cancer (CRC) is a commonly diagnosed cancer with a high fatality rate around the world [1]
LINC01224 level is increased and activated by Yin Yang 1 (YY1) in colorectal cancer (CRC) To study the importance of LINC01224 in CRC, the expression change of LINC01224 was detected
LINC01224 level was elevated in colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ) in the The Cancer Genome Atlas (TCGA) database (Fig. 1A)
Summary
Colorectal cancer (CRC) is a commonly diagnosed cancer with a high fatality rate around the world [1]. Exploring novel targets for CRC therapy is necessary. Long noncoding RNAs (lncRNAs; >200 nucleotides) with diverse cellular functions and lacking proteincoding potential are related to human cancer progression [3]. LncRNAs have become potential targets for the treatment of CRC [4]. The roles played via lncRNAs in CRC might be associated with the interaction of microRNAs (miRNAs) and mRNAs [5]. The lncRNA LINC01224 exhibits an oncogenic role in multiple human malignancies [6,7,8,9], including CRC [10]. The repertoire of LINC01224 in CRC progression is largely unclear. Long noncoding RNAs (lncRNAs) are related to colorectal cancer (CRC) development. The role and mechanism of lncRNA LINC01224 in CRC development are largely unknown
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