Abstract
BackgroundLong non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. Nonetheless, the role of LINC01158 in glioma remains to be elucidated.MethodsqRT-PCR, western blot and GEPIA database were applied for reporting the expression of CENPK and LINC01158 in glioma and the correlation between LINC01158 and CENPK expression. EdU, colony formation, CCK-8, caspase-3 activity and TUNEL assays probed the impacts of LINC01158 on glioma cell growth. Subcellular fractionation and FISH assays revealed the cellular distribution of LINC01158. Luciferase reporter and RIP assays examined ceRNA network of LINC01158, CENPK and miR-6734-3p.ResultsLINC01158 and CENPK were both overexpressed in glioma and a positive regulation of LINC01158 on CENPK was corroborated. LINC01158 served a pro-proliferative and anti-apoptotic part in glioma by sponging miR-6734-3p to augment CENPK.ConclusionLINC01158 enhances CENPK by serving as sponge for miR-6734-3p to facilitate glioma development, proposing LINC01158 as a new player in glioma.
Highlights
Long non-coding RNAs have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature
We discovered that Centromere protein K (CENPK) and LINC01158 were both increased in glioma cells and tissues, and positively correlated with each other
LINC01158 positively regulated CENPK and both were overexpressed in glioma The enrichment of LINC01158 was probed and a dramatic increase of LINC01158 in GBM tissues was illustrated by the data from GEPIA database (Fig. 1a)
Summary
Long non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. In China, brain tumor is one of the top ten most common cancers [1, 2]. As elucidated from the data of human genomic sequence, only 2% transcripts have potent capacities of protein encoding, and the rest are noncoding RNAs (ncRNAs). NcRNAs can be primarily divided into two subtypes, including long noncoding RNAs (lncRNAs) and microRNAs (miRNAs), both of which exert multibiological functions in the development of tumors [5, 6]. LncRNAs or miRNAs could be either oncogenic or tumor-suppressive during the onset and progression of cancers [7, 8].
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