Abstract
BackgroundLong intergenic non-coding RNA 01094 (LINC01094) is probably a novel regulator in cancer biology. This study aimed to probe into the function and mechanism of LINC01094 in ovarian cancer (OC).MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) assay was utilized to measure LINC01094 and miR-577 expressions in OC tissues and cell lines. Western blot was used to examine the expressions of epithelial-mesenchymal transition (EMT)-related proteins, β-catenin, c-Myc and cyclin D1. Cell counting kit-8 (CCK-8) and Transwell assays were used to detect the proliferation, migration and invasion of SKOV3 and 3AO cells, respectively. Eventually, dual-luciferase reporter gene assay was employed to detect the regulatory relationship between miR-577 and LINC01094.ResultsLINC01094 expression was elevated in OC tissues and cell lines. High LINC01094 expression was associated with higher FIGO stage, lymph node metastasis and the shorter overall survival rate in patients with OC. Meanwhile, LINC01094 knockdown inhibited OC cell proliferation, migration, invasion and EMT. In addition, miR-577 was demonstrated to be a direct downstream target of LINC01094 in OC and inhibition of miR-577 reversed the biological effects of LINC01094 knockdown on OC cells. Additionally, LINC01094 / miR-577 axis regulated the expressions of β-catenin, c-Myc and cyclin D1 in OC cells.ConclusionLINC01094 promotes the proliferation, migration, invasion and EMT of OC cells by adsorbing miR-577.
Highlights
Ovarian cancer (OC) is one of the deadliest cancers among women worldwide, triggering about 151,900 deaths per year [1, 2]
LINC01094 expression was elevated in ovarian cancer (OC) tissues and cell lines First of all, Gene Expression Profiling Interactive Analysis (GEPIA) database manifested that LINC01094 was differentially expressed in OC tissues and adjacent normal tissues, and LINC01094 expression in cancer tissues was remarkably higher than that in normal tissues (Fig. 1a)
QRT-PCR assay showed that the expression of LINC01094 in the collected OC samples was markedly higher in comparison with that in adjacent nontumor tissues (Fig. 1b)
Summary
Ovarian cancer (OC) is one of the deadliest cancers among women worldwide, triggering about 151,900 deaths per year [1, 2]. Long non-coding RNA (LncRNA) has been one of the research hotspots in recent years. It is found that multiple lncRNAs are abnormally expressed in cancer cells, which modulate carcinogenesis and cancer progression [7]. A recent study reports that long intergenic noncoding RNA 01094 (LINC01094) is transcriptionally activated by FOXM1 and it promotes the progression of clear cell renal cell carcinoma [8]. In cancer biology, the role of LINC01094 has not been fully elucidated. Long intergenic non-coding RNA 01094 (LINC01094) is probably a novel regulator in cancer biology. This study aimed to probe into the function and mechanism of LINC01094 in ovarian cancer (OC)
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