Abstract
BackgroundPancreatic cancer (PC) is one of the deadliest cancers about the digestive system. Recent researches have validated that long non-coding RNAs (lncRNAs) play vital roles in various cancers, while the function of LINC01006 in PC is rarely clarified.Aim of the studyInvestigation of the specific role of LINC01006 in PC.MethodsLINC01006 expression was examined by RT-qPCR. CCK-8, EdU, transwell, wound healing, and western blot assays were carried out to explore the function of LINC01006 in PC. The interaction among LINC01006, miR-2682-5p and HOXB8 was verified by luciferase reporter, RIP and ChIP assays.ResultsThe expression of LINC01006 was markedly upregulated in PC tissues and cells. Furthermore, LINC01006 knockdown inhibited PC cell proliferation, invasion and migration, and upregulation of LINC01006 led to the opposite results. Besides, miR-2682-5p expression was downregulated and negatively regulated by LINC01006 in PC. Meanwhile, LINC01006 could bind with miR-2682-5p in PC. Moreover, miR-2682-5p negatively regulated HOXB8 expression and there was a binding site between miR-2682-5p and HOXB8 in PC. Additionally, miR-2682-5p overexpression or HOXB8 knockdown rescued the promotive effects of LINC01006 upregulation on PC cell progression. Similarly, miR-2682-5p inhibition or HOXB8 overexpression countervailed the repressive role of LINC01006 downregulation in PC cell progression. In addition, the transcription factor HOXB8 could activate LINC01006 transcription in PC.ConclusionsLINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis, which may facilitate the treatment for PC.
Highlights
Pancreatic cancer (PC) is one of the deadliest cancers about the digestive system
LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/homeobox B8 (HOXB8) axis, which may facilitate the treatment for PC
LINC01006 is highly expressed in PC and promotes the proliferation and metastasis of PC cells A previous long non-coding RNAs (lncRNAs)-seq was conducted to reveal the differentially expressed lncRNA in PC tissues compared with normal ones
Summary
Pancreatic cancer (PC) is one of the deadliest cancers about the digestive system. Recent researches have validated that long non-coding RNAs (lncRNAs) play vital roles in various cancers, while the function of LINC01006 in PC is rarely clarified. Unlike other cancers whose survival rates were improved, the incidence and mortality of pancreatic cancer (PC) both keep on increasing [1, 2]. Zhang et al Cancer Cell Int (2019) 19:320. LncRNA CRNDE/miR-181a-5p/Wnt/βcatenin signaling pathway promotes the occurrence of colorectal cancer [15]. LncBRM activates YAP1 signaling to promote self-renewal of stem cells in liver cancer [17]. LncRNA HOTAIR sponges miR-613 to promote cell proliferation and metastasis in PC through targeting notch3 [20]. Despite the fact that LINC01006 has been discovered to participate in gastric cancer progression [21], its influences in PC sustain unclear
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