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Abstract
Bladder cancer has easy recurrence characteristics, but its occurrence and development mechanism are still unclear. Non-coding RNA is a kind of RNA that exists widely and cannot be translated into proteins, which has played a key role in the regulation of biological functions of tumor cells. However, the regulation mechanism of non-coding RNA on bladder tumors is not fully understood. By microarray analysis and database analysis, we found that LINC00511 was significantly highly expressed in bladder cancer. The expressions of LINC00511, miR-143-3p, and PCMT in bladder cancer tissues and cells were detected by quantitative reverse transcription–polymerase chain reaction. The relationship between the expressions of miR-143-3p and PCMT1 and the clinicopathological parameters of the tumor was analyzed. The proliferation and invasion of bladder cancer cells were detected by MTT assay and Transwell assay. The expression levels of E-cadherin and vimentin in bladder cancer cells were detected by Western blot. Cell apoptosis was detected by flow cytometry. In vivo, TCCSUP or SW780 cells were inoculated into BALB/c nude mice to detect tumor volume and weight. Bioinformatics and dual luciferase reporter gene were used to analyze the relationship between LINC00511 and miR-143-3p and its downstream target gene PCMT1. The results showed that LINC00511 could target miR-143-3p/PCMT1 to regulate the proliferation, migration, and apoptosis of bladder cancer TCCSUP or SW780 cells and promote the occurrence and development of bladder cancer.
Highlights
Bladder cancer is a kind of urogenital tumor with high morbidity and mortality (Chen et al, 2020; Wołacewicz et al, 2020)
The clinical samples collected through PCR detection found that LINC00511 was elevated in the cancer tissues compared to the adjacent tissues (Figure 1B)
It has been reported that knockdown of LINC00511 promoted apoptosis of bladder cancer cells via suppressing Wnt/β-catenin signaling pathway (Li et al, 2018)
Summary
Bladder cancer is a kind of urogenital tumor with high morbidity and mortality (Chen et al, 2020; Wołacewicz et al, 2020). Radical surgery and radiotherapy are effective, one-quarter of patients with muscle-invasive bladder cancer still have a poor prognosis (Sternberg et al, 2015). Approximately 75% of patients presented with non-muscular invasive bladder cancer, and the other 25% presented with. PCMT1 in Bladder Carcinoma Cells muscular invasive bladder cancer or metastatic tumor (Burger et al, 2013). Patients with non-muscular invasive bladder cancer have up to 50% risk of recurrence and 20% risk of progression within 5 years. Cystoscopy is an effective method for detecting bladder cancer, it can produce several adverse reactions. It is indispensable to develop noninvasive diagnostic tools for the screening and monitoring of bladder cancer to improve the quality of life of patients
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