Abstract
BackgroundLinc00152 has been identified highly associated with the tumorigenesis and development of gastric cancer, however, the detailed mechanism of Linc00152 involved still remains unclear.MethodsRT-PCR and western blot were used to detect the expression of Linc00152 and EGFR. The CCK8 and EDU assay was employed to measure cell proliferation while xenotransplantation technology was applied in BALB/C nude mice. The interaction between lncRNA and target protein was investigated by RNA pull-down and RNA immunoprecipitation assay.ResultsIn this study, we first confirmed the upregulation of cytoplasmic expressed Linc00152 in 72 pair tissues of gastric patients. A suppression of cell proliferation and tumor growth was obtained in MGC803 and HGC-27 cells treated with Linc00152 shRNA. RNA pull-down and RIP assay revealed that Linc00152 could directly bind with EGFR which caused an activation of PI3K/AKT signaling.ConclusionWe first found that Linc00152 could promote tumor growth through EGFR-mediated PI3K/AKT pathway which may serve as potential targets for therapy in the future.
Highlights
Linc00152 has been identified highly associated with the tumorigenesis and development of gastric cancer, the detailed mechanism of Linc00152 involved still remains unclear
We mainly focused on the reported Long noncoding (lncRNA) entitled Linc00152
We found that MGC803 and HGC-27 was expressed with a high abundance (Fig. 1b)
Summary
Linc00152 has been identified highly associated with the tumorigenesis and development of gastric cancer, the detailed mechanism of Linc00152 involved still remains unclear. Despite recent improvements in surgery and chemotherapy, gastric cancer remains a very high morbidity and mortality [3, 4]. Among them are long noncoding RNAs (lncRNAs) with the transcripts of greater than 200 nucleotides with no or little protein coding function [8]. Recent studies have identified multiple functional effects of lncRNAs involving in multiple progression of human cancers including regulating gene expression through modulation of chromatin remodeling, controlling of gene transcription, posttranscriptional mRNA processing, protein function or localization and intercellular signaling [9,10,11]. Researchers identified that several lncRNAs could be modified epigenetically including methylation, ubiquitination, miRNA-induced regulation though a network [12, 13]
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