Abstract
The present work aimed to probe into the effect of long non-coding RNA (lncRNA) LINC00152 on gastric cancer (GC) cells proliferation by regulating miR-193a-3p and its target gene MCL1. Transfected si-LINC00152 was used to down-regulate LINC00152, and cells proliferation was measured by the cell counting kit-8 (CCK-8) assay. Cell apoptosis and cell cycle were analyzed by flow cytometry (FCM). Besides, we also detected the potential functional effects of differential expression of LINC00152 in vivo using nude mouse xenograft model. We overexpressed and downexpressed miR-193a-3p to study the in vitro effect of miR-193a-3p on GC cells proliferation and vitality. And MCL1 was silenced by shRNA to investigate the effect of MCL1 on proliferation of GC cells. In this research, LINC00152 was proven to have a higher expression level in GC tissues than in the adjacent normal tissues. GC cells proliferation was inhibited after LINC00152 was down-regulated. LINC00152 inhibited the expression of miR-193a-3p, which negatively regulated MCL1. In addition, GC cells proliferation was inhibited by cell transfection with shRNA-MCL1, and enhanced by transfection with miR-193a-3p mimics. Our study suggested that LINC00152 was overexpressed in GC tissues, and it down-regulated miR-193a-3p to enhance MCL1 expression thereby promoting GC cells proliferation.
Highlights
Amongst the most critical malignant neoplasms, gastric cancer (GC) is the second common leading cause of deaths relevant to cancer around the world [1]
According to the microarray analysis, 126 long non-coding RNA (lncRNA) were overexpressed and 82 lncRNAs were lowly expressed in the GC tissues, as shown in the volcano plot (Figure 1A) and the heat map (Figure 1B)
The flow cytometry (FCM) assay was used to examine cell cycle progression, AGS and BGC-823 cells transfected with si-LINC00152 showed a cell cycle arrest at G1/G0 phase (P
Summary
Amongst the most critical malignant neoplasms, gastric cancer (GC) is the second common leading cause of deaths relevant to cancer around the world [1]. We worked over the relationship between LINC00152 expression and the cell multiplication of GC, and we verified both in vivo and in vitro that LINC00152 promoted gastric cells proliferation by regulating miR-193a-3p and its target gene MCL1. MCL1-WT and MCL1-mut sequences were inserted into AGS and BGC-823 cells by the pGL3 plasmid, co-transfected with miR-193a-3p mimics or mimics NC. Data were expressed in the format of mean +− S.D., and Student’s t test c 2018 The Author(s)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
