Abstract

1. Excised corn root tips grown in a liquid medium (solution C) composed of calcium nitrate, magnesium sulphate, potassium dihydrogen phosphate, ferric chloride, and dextrose showed limited growth and sometimes became abnormal. Similar results were secured in a modified Pfeffer's solution containing dextrose. 2. The amount of growth in solution C varied with the brand of dextrose used, even when the dextrose was of high purity. 3. The substitution of levulose, cane sugar, xylose, or maltose for dextrose in solution C was of no benefit; in fact, the growth with xylose and maltose was very poor. 4. Growth was improved by the addition to solution C of Difco agar, water extracts of agar, qualitative filter paper, water extracts of qualitative filter paper, soluble starch, autolyzed or dried brewers' yeast, or a mixture of salts of manganese, boron, and zinc. 5. The best results were secured by the add tion of qualitative filter paper in the proportion of one half sheet, 9 cm. in diameter, to 30 cc. of medium....

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