Abstract
Objective: Men with nonobstructive azoospermia (NOA) may have sperm retrieved from testicular tissue for use in intracytoplasmic sperm injection (ICSI). While cryopreserved sperm from men with obstructive azoospermia have been used for ICSI as effectively as fresh sperm in this population, it is unclear if the low numbers of sperm retrieved from men with NOA survive the freeze/thaw process sufficiently for use in ICSI. We have assessed the effect of freeze/thaw on sperm from men with NOA and present the results of ICSI using frozen-thawed testicular sperm in NOA.
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