Year-end Sale % OFF 
Abstract
SummaryMicrobiome plays a key role in determining soil suppressiveness against invading pathogens. Our previous study revealed that microbial community of bulk soil could be manipulated by lime and ammonium bicarbonate fumigation followed by biofertilizer application. However, the assembly of microbial community suppressive to banana Panama disease in the rhizosphere is still unclear. In this study, we used high‐throughput sequencing and quantitative PCR to explore the assembly of rhizosphere microbiome associated with banana Panama disease suppression in a two‐seasonal pot experiment. We found biofertilizer applied to lime and ammonium bicarbonate fumigated soil significantly (P < 0.05) reduced the abundance of rhizosphere Fusarium oxysporum compared to biofertilizer applied to non‐fumigated soil. Principal coordinate analysis revealed that biofertilizer applied to lime and ammonium bicarbonate fumigated soil re‐shaped the rhizosphere bacterial community composition by increasing the phylogenetic relatedness, and stimulating indigenous microbes, for example, Gemmatimonas, Sphingomonas, Pseudomonas, Lysobacter and Bacillus. Co‐occurrence analysis revealed that potential species involved in disease suppression were more interrelated in disease‐suppressive soils. Taken together, lime and ammonium bicarbonate fumigation followed by biofertilizer application could induce banana rhizosphere to assemble beneficial microbes dominated consortia to suppress banana Panama disease.
Highlights
Interactions between microbes in bulk and rhizosphere soil, and plant provide a potential to dynamically affect plant health and production through complex feedback mechanisms (Chaparro et al, 2012)
We found biofertilizer applied to lime and ammonium bicarbonate fumigated soil significantly (P < 0.05) reduced the abundance of rhizosphere Fusarium oxysporum compared to biofertilizer applied to non-fumigated soil
Among the soils sampled at the end of pot experiment during the second season, the treatment of biofertilizer applied to lime and ammonium carbonate fumigated soil (LAB) with lower disease incidence showed a significantly lower abundance of F. oxysporum in the rhizosphere soils than that in the treatment of biofertilizer applied to non-fumigated soil (CKB) (Fig. 1A)
Summary
Interactions between microbes in bulk and rhizosphere soil, and plant provide a potential to dynamically affect plant health and production through complex feedback mechanisms (Chaparro et al, 2012). Since rhizosphere microbiota acts as the first line of plant defence against soil-borne pathogens (Cook et al, 1995; Philippot et al, 2013), the study of how plant recruits beneficial microbes from bulk soil to assemble a disease-suppressive microbiome in the rhizosphere could provide an important understanding of plant–soil–microbe interactions associated with soil-borne disease suppression. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
