Abstract
BackgroundLimb remote ischemic postconditioning (LRIP) can ameliorate cerebral ischemia–reperfusion injury (IRI), while the underlying mechanism remains elusive. Hypoxia-inducible factor 1α (HIF-1α) is an important transcription factor during cerebral ischemia damage. However, whether the neuroprotective effect of LRIP could be associated with HIF-1α is somewhat unclear. Here we tested the hypothesis that Limb remote ischemic postconditioning (LRIP) protecting brain from injury in middle cerebral artery occlusion (MCAO) rat model was associated with HIF-1α expression.ResultsLRIP was conducted with 3 cycles of 10 min occlusion/10 min reperfusion at the beginning of reperfusion. The analysis of neurobehavioral function and triphenyltetrazolium chloride (TTC) staining showed the neurological deficit, brain infarct and cerebral edema, caused by ischemia–reperfusion injury (IRI), were dramatically ameliorated in LRIP administrated animals. Meanwhile, the result of Q-PCR and western blot revealed that the overexpression of HIF-1α induced by IRI could be notably suppressed by LRIP treatment.ConclusionsLRIP exhibits a protective effect against cerebral ischemia/reperfusion and the possible mechanism is associated with the suppression of HIF-1α in stroke rats.
Highlights
Limb remote ischemic postconditioning (LRIP) can ameliorate cerebral ischemia–reperfusion injury (IRI), while the underlying mechanism remains elusive
We examined the neuroprotective effect of LRIP using middle cerebral artery occlusion (MCAO) rat model, indicated by neurological deficit score, infarct volume and brain edema
These findings showed that the postconditioning treatment could improve the neurological function caused by MCAO injury
Summary
Limb remote ischemic postconditioning (LRIP) can ameliorate cerebral ischemia–reperfusion injury (IRI), while the underlying mechanism remains elusive. Whether the neuroprotective effect of LRIP could be associated with HIF-1α is somewhat unclear. We tested the hypothesis that Limb remote ischemic postconditioning (LRIP) protecting brain from injury in middle cerebral artery occlusion (MCAO) rat model was associated with HIF-1α expression. No evidence is to address the role of HIF-1α in brain ischemia rats subjected to LRIP. We examined the neuroprotective effect of LRIP using MCAO rat model, indicated by neurological deficit score, infarct volume and brain edema. Using the analysis of Q-PCR, western blot and immunohistochemistry, we detected whether HIF-1α was involved in ischemic brain subjected to LRIP
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