Lignocellulolytic enzyme potential of dark septate endophyte (dse) from Pinus merkusii roots in Dramaga Bogor Indonesia

Abstract

The ability of Dark Septate Endophyte (DSE) derived from the roots of P. merkusii in decomposing organic matter is not much published. This study aims to determine the activity of lignolytic and cellulolytic enzymes, as well as the value of the cellulolytic index of Apls, isolates from P. merkusii roots taken from IPB Dramaga Bogor. Enzyme activity was tested by measuring the clear zone formed, while the cellulolytic index value was obtained by measuring the ratio of the clear zone formed around the colony. Data were analyzed descriptively. The results identified 10 isolates, 8 Apls isolates had potential enzyme activity that able to form clear zone, 5 isolates had lignolytic enzyme activity (Apls 1.4.1, Apls 2.3.1.1, Apls 3.4.2, Apls 2.1.1 and Apls 3.4 1.1) and 1 isolate had cellulolytic enzyme activity (Apls 3.1.3), and 2 isolates had lignocellulolytic enzyme activity (Apls 1.5.3 and Apls 3.4.3b), with the highest cellulolytic index value in Apls 1.5.3 isolates of 0,87 mm. 2 isolates had not lignocellulolytic enzymes activity (Apls 3.4.3 and Apls 3.1.4). The DSE roots of P. merkusii from IPB Dramaga Bogor had the potential for lignocellulolytic enzymes of 20 per cent of the total DSE found.