Abstract
In this work we investigated the enzymes produced by Bacillus aryabhattai BA03, particularly in those involved in ligninolytic activities such as laccases (Lac), lignin peroxidases (LiP) and Mn-dependent peroxidase (MnD-P). In this way, the maximal production of Lac (0.069 ± 0.000 U/mL) was obtained at pH 9, and 37 ºC after 72h. LiP expressed the highest activity at 96h in acid medium at 37 ºC (0.741 ± 0.029 U/mL). Meanwhile, the strain produced MnD-P (1.052 ± 0.001 U/mL) at the highest temperature assayed (44 ºC) and pH 7 at 72h. In addition, this microorganism produced resistant endospores able to germinate after the sterilization program (121 ºC, 15min) showing a high enzymatic activity. Using the heat-treated culture as inoculum, the percentage of decolorization of 150mg/L of Coomassie Brillant Blue reached 89.42 ± 0.11% in only 24h. These results open the use of these enzymes and endospores in bioremediation processes carried out under different temperatures and pH values.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.