Abstract
Differences in levels of lignin in the plant between conventional and transgenic cultivars RR has been reported by several authors, however, there are few studies evaluating the influence of spraying of glyphosate on the lignin in the plant and RR soybean seeds. The aim of this study was to evaluate the physiological quality of RR transgenic soybean seeds and the lignin contents of plants sprayed with the herbicide glyphosate. The assays were conducted both in greenhouse and field in the municipality of Lavras, MG, in the agricultural year 2007/08. The experiment was arranged in a splitplot design with four replicates, considering the treatments hand weeding and herbicide glyphosate as plots, and five RR soybean cultivars (BRS 245 RR, BRS 247 RR, Valiosa RR, Silvânia RR and Baliza RR) as splitplots. In the greenhouse, the cultivars tested were BRS 245 RR and Valiosa RR in a randomized block design with four replicates. The sprayings were carried out at stages V3, V7 and early R5 (3L/ha). The 1000 seed weight, mechanical injury, germination and germination velocity index, emergence velocity index, accelerated aging, electrical conductivity and water soaking seed test, lignin content in the seed coat, in the stem and legumes were determined. The spraying of glyphosate herbicide, in greenhouse and field, did not alter the physiological quality of seeds and the lignin contents in the plant.
Highlights
Lignification of the plant and seed quality of RR soybeans sprayed with herbicide glyphosate
Differences in levels of lignin in the plant between conventional and transgenic cultivars RR has been reported by several authors, there are few studies evaluating the influence of spraying of glyphosate on the lignin in the plant and RR soybean seeds
The aim of this study was to evaluate the physiological quality of RR transgenic soybean seeds and the lignin contents of plants sprayed with the herbicide glyphosate
Summary
Foram conduzidos dois experimentos de produção de sementes, um em campo e outro em casa de vegetação, no ano agrícola 2007/08, na Universidade Federal de Lavras, em solo classificado como Latossolo Roxo distroférrico, fase cerrado. Nas parcelas em que o controle de plantas daninhas foi realizado com o herbicida, utilizou-se o produto comercial Roundup Ready©, princípio ativo glifosato, na dosagem de 3L/ha, sendo realizadas três pulverizações nos estádios de desenvolvimento V3, V7 e início de R5, segundo Fehr & Caviness (1977). Para as análises e determinações, foi utilizada a mistura das sementes retidas nas peneiras de crivo circular 5,55 mm e 6,35 mm, sendo que, para os testes fisiológicos, com exceção do teste de condutividade elétrica, as sementes foram tratadas com o fungicida Vitavax Thiran 200 SC, na dosagem de 250 mL/100 kg de sementes. Inicialmente, foi aplicado o teste F (Storck et al, 2000) e, quando verificado efeito significativo dos tratamentos, realizado o teste de contraste de médias Scheffé entre os tratamentos com e sem pulverização (ensaio em casa de vegetação) e capina x herbicida (ensaio de campo), e o teste de médias Scott-Knott entre as cultivares de soja
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