Abstract
Lignan Glucosides from Roots of Urtica dioicaLignan glucosides isolated from stinging nettle roots (Urtica dioica) were separated by GC on short glass capillary columns (5–6 m) after appropriate derivatization. By use of mass spectrometry (GC/EI‐MS, EI‐MS, LSI‐MS) and NMR spectroscopy, 11 lignan glucosides were identified, 5 of which were previously unknown. Mixtures of isomeric lignan glucosides which could not be separated by GC were investigated by two‐dimensional NMR methods. The spectra allowed the identification of the single components. Mass spectra of the trimethylsilylated glucosides show typical fragments which enabled us to decide if the glucose was attached to an alcoholic or phenolic hydroxy group of the aglycone. The nature of the aglycones could be also deduced from the spectra.
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