Abstract
In this study, four new lignan glucosides, named difengpiosides A–D (1–4), were isolated from the stem barks of Illicium difengpi, together with seven known compounds 5–11. Their structures were identified on the basis of spectroscopic analyses (1D and 2D NMR, HRESIMS, CD) and a comparison with literature data. All the compounds were evaluated for their inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells.
Highlights
The EtOH extract of the stem barks of I. difengpi were suspended in water and successively partitioned with petroleum ether, EtOAc, and n-BuOH
The n-BuOH-soluble extract was subjected to multiple chromatographic fractionations using silica gel, Sephadex LH-20, and ODS, yielding compounds 1 ́11 (Figure 1)
−O−CH−CH−CH2O− spin systems, which were confirmed by the 1H-1H COSY spectrum (Figure 2)
Summary
Its stem bark is listed in the Chinese Pharmacopeia as a traditional Chinese medicine to treat rheumatic arthritis [1]. Previous phytochemical studies on this plant mainly focused on the low and middle polarity components and reported the isolation of over 40 compounds, including phenylpropanoids, lignans, triterpene acids, sesquiterpenes and others [2,3,4,5,6,7]. There are few investigations on the polar substances of I. difengpi. I. difengpi, the present study describes the isolation and structure elucidation of four new lignan glycosides together with seven known compounds from the n-BuOH-soluble fraction of an EtOH extract of the stem barks of this plant, as well as their inhibitory activities against nitric oxide production in lipopolysaccharide-induced RAW264.7 cells
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