Abstract
Lighting up Neuroanatomy.
Highlights
“Contemporary neuroscience is in urgent need of a new generation of neuroanatomical techniques allowing scalable, reliable, specific, and quantitative analysis of macroscopic portions of brain tissue with cellular or sub-cellular resolution.”
In confocal light sheet microscopy (LSM), the approach used by Silvestri et al, the sample is illuminated with a thin sheet of light and optically sectioned, typically in a wide-field detection scheme
LSM entails a number of interrelated steps, which are clearly detailed in Silvestri et al As shown in their figure 1, the experimental pipeline for large-volume quantitative neuroanatomy consists of tissue clearing, actual imaging, image stitching, automatic cell localization, and further statistical analysis
Summary
A commentary on Quantitative neuroanatomy of all Purkinje cells with light sheet microscopy and high-throughput image analysis by Silvestri, L., Paciscopi, M., Soda, P., Biamonte, F., Iannello, G., Frasconi, P., and Pavone, F. “Contemporary neuroscience is in urgent need of a new generation of neuroanatomical techniques allowing scalable, reliable, specific, and quantitative analysis of macroscopic portions of brain tissue with cellular or sub-cellular resolution.” In this first sentence of their Discussion, Silvestri et al (2015) succinctly set forth the key issues of their contributing article; namely, visualization of Purkinje cells in 3-D volumes, and the methods for doing so.
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