Abstract

Light-induced absorbancy changes in the near-infrared wavelength region in intact cells and cell free preparations of photosynthetic bacteria have been object of several investigations ( Duysens, 1952; Duysens et al., 1956; Arnold and Clayton, 1960; Clayton, 1962; Vredenberg and Duysens, 1963; Sybesma and Vredenberg, 1963, 1964; Kuntz et al., 1964). An absorption decrease around 870–890 mμ in purple bacteria has been attributed to the oxidation of a reaction center P 890 upon illumination. Recently Clayton (1963) reported in Rhodopseudomonas spheroides a different type of infrared absorption changes. These changes, which are not observed in Rhodospirillum rubrum (e.g. Duysens et al., 1956), could not be attributed to the oxidation of P 890, because of their kinetics and sign at 890 mμ. This paper gives the results of a closer study of the characteristics of these changes in various species of sulphur and non sulphur purple bacteria. In contradistinction to Clayton we conclude that they are not caused by a primary light reaction.

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