Abstract
We present a implementation method of light-sheet microscopy utilizing a highly miniaturized device that produces light-sheet illumination while immersed in the sample container. Our miniaturized plane illuminator (MPI) internally equips a two-axis beam-scanning mechanism based on a magnetostatically driven optical fiber cantilever. A light sheet is produced by fast scanning of the focused beam in an axis while the illumination plane can move in the other axis for positioning and 3D imaging. Our MPI device is so compact in a 1.5 mm-thick needle form that it can be conveniently placed in the right vicinity of the imaging sample. Because the illumination is directly given in the sample-surrounding medium, a great deal of operational flexibility is obtained with an uncompromised beam quality. We could build a light-sheet microscopy system with a conventional inverted microscope frame by attaching our MPI upgrade kit as an add-on module. In this study, the optical and electromechanical characteristics of our MPI device were carefully investigated. As well, light-sheet microscopy imaging of various samples was performed to validate the practical power of our technique. We found our MPI can provide a low-cost and easy-to-use imaging mode, and make the light-sheet microscopy more available in various applications.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
