Abstract
The last decade has seen tremendous development in LST, specifically in LSFM. This has resulted in a plethora of imaging techniques, each equipped with new features (such as thin light sheet, large FOV, oblique light sheet, and Bessel light sheet) most of which are driven by applications. The development of light sheet illumination and its integration with orthogonal detection is a demanding task that requires precision instrumentation of the entire imaging system. Some of the popular variants of LSFM include OPFOS, DLSM, TLSM, MLSM, SCAPE, and LLSM. Moreover, the emergence of super-resolution analog and multiphoton variant of LSM has contributed toward a near-complete imaging system modality. This chapter is dedicated to several exciting variants of LSM/LSFM and discusses its immense potential in biological imaging. Indeed, the emergence of LST has ushered in a new era for microscopy and imaging.
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