Abstract
We present a light sheet fluorescence microscope dedicated to image “Organ-on-chip”-like biostructures in microfluidic chip. Based on a simple design, the setup is built around the chip and its environment to allow 3D imaging inside the chip in a microfluidic laboratory. The experimental setup, its optical characterization and first volumetric images are reported.
Highlights
Nowadays, the organ-on-chip technology presents a large interest for biomedical researches
Light Sheet Fluorescence Microscopy (LSFM) seems to be a promising solution to analyze the inner parts of samples
Contrary to other studies focusing on super-resolution or large field of view, the main purpose of this instrument is to obtain the best compromise between large field of view and resolution that can be achieved to morphologically analyze samples
Summary
The organ-on-chip technology presents a large interest for biomedical researches Important issues, such as the vascularization of organoids or tissues, remain and must be solved to enhance the potential of this application technique. LSFM is an optical imaging technique that has been increasingly used in biological applications ranging from molecular biology to whole mount tissue analysis. This technique presents a lot of advantages in microdevice field[1,2,3,4,5]. The main advantages of this technique are the high-speed volumetric acquisition rate and the low photo-toxicity[6,7,8] These two particularities are essential for rapid 3D and for 4D imaging
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