Abstract
A theory is presented showing that the effect of a very small amount of high molecular weight impurity on a globular protein solution is to decrease the measured diffusion constant and increase the weight average molecular weight in equal proportions. This applies when the diffusion constant is determined by a light scattering Rayleigh linewidth measurement using the autocorrelation method. Experimental verification of this is provided by the results of measurements upon a solution of bovine plasma albumin containing a high molecular weight impurity. The effects of different amounts of impurity were simulated by making measurements at different angles of scatter. The limitations of the theory are discussed.
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