Abstract
An experimental study was carried out, aimed at optimizing the opto-geometric configuration for measuring the concentration of biological cells by means of static light scattering measurements. A LED-based optoelectronic setup making use of optical fibers was experimented, as the precursor of a low-cost device to be integrated in instrumentation for cytometry. Two biological sample types were considered as test samples of the most popular analyses - cervical cells and urine, respectively. The most suitable wavelengths and detecting angles were identified, and calibration curves were calculated.
Highlights
Liquid-based cytology (LBC) is an innovative way of preparing biological samples for cytological examinations in the laboratory
This paper presents the results of an experimental nephelometric study which was performed on cervical and urine cells in a CYTOfast® solution
A glass vial containing 32 ml biological sample was inserted in a jig
Summary
Liquid-based cytology (LBC) is an innovative way of preparing biological samples for cytological examinations in the laboratory. It consists of fixing the collected sample in a preservative alcohol-based fluid for further clarification, centrifugation, and depositing a thin layer of cells on a slide. Since 1999, Hospitex Diagnostics srl has been implementing an innovative and effective proprietary LBC method, the CYTOfast® system [9]. It makes use of CYTOfast® solution, a universal preservative solution that makes it possible to preserve the physiological structure and morphology of any kind of cell for 24 months at room temperature. The most suitable wavelengths and detecting angles were identified, and calibration curves were calculated
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