Light‐scattering and size‐exclusion chromatographic characterization of hydroxyethyl cellulose acetate

Abstract

AbstractA recently developed analytical method of combining off‐line laser light scattering (LLS) and size exclusion chromatography (SEC) was used to investigate a set of moderately distributed hydroxyethyl cellulose acetate (HECA) samples in tetrahydrofuran (THF) at room temperature. Our results have shown that this new LLS + SEC method is suitable for the characterization of molecular weight distribution of HECA. By using this method, we have simultaneously determined two calibrations of V (cm3) = 45.3 − 1.89 log (M) and D (cm2/s) = 2.45 × 10−4 M−0.60, where M is the molecular weight of HECA; V, the elution volume in SEC; and D, the translational diffusion coefficient in dynamic LLS. In addition, our results have also indicated that the chain conformation of HECA in THF at room temperature is a slightly extended linear coil. © 1995 John Wiley & Sons, Inc.