Light-regulated compensation of wat1 (walls are thin1) growth and secondary cell wall phenotypes is auxin-independent

Abstract

We previously reported the characterization of walls are thin1 (wat1), an Arabidopsis mutant that exhibits two developmental phenotypes in stems: (i) a severe decrease in fiber secondary cell wall thickness and (ii) a reduction in stem height.1 Auxin concentration and transport were also significantly reduced in the stem base of wat1 plants. In the original study, these characteristics were observed in plants grown under short day conditions (9h light /15h dark). Herein, we provide evidence for partial phenotypic complementation of both wat1 developmental phenotypes when grown under a continuous light regime. Interestingly, when auxin concentration and basipetal transport were measured in these plants, neither was restored to wild type levels. These results suggest that free auxin concentration is not responsible for the partial light–regulated complementation of wat1-mediated phenotypes.