Light Quality Affects Growth and Physiology of Carpesium triste Maxim. Cultured In Vitro

Jin Zhao,Yoo Gyeong Park,Byoung Ryong Jeong,Luc The Thi

doi:10.3390/agriculture10070258

Jin Zhao, Yoo Gyeong Park + Show 2 more

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https://doi.org/10.3390/agriculture10070258

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Journal: Agriculture	Publication Date: Jul 2, 2020
Citations: 20	License type: CC BY 4.0

Affiliation: Gyeongsang National University

Abstract

The objective of this study was to investigate the effect of light quality on the growth and contents of photosynthetic pigments and total flavonoids, the parameters of chlorophyll a fluorescence, antioxidant capacities, and enzyme activities. It was intended to preliminarily explore the internal mechanisms involved for the effect of light quality on the growth and physiology of Carpesium triste Maxim. Stem apex explants were cultured on the Murashige and Skoog (MS) medium under white (W), red (R), blue (B), or a 1:1 mixture of red and blue (RB) light-emitting diodes (LEDs) for four weeks. The RB induced sturdy plantlets and increased the contents of photosynthetic pigments and total flavonoids, photosynthetic electron transport and efficiency, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity, and activities of antioxidant enzymes in the plantlets. Taken together, combinations of monochromatic red and blue LEDs could be used for the best production of high-quality C. triste plantlets in vitro.

Highlights

Carpesium triste Maxim. is a rare perennial herbal species of the Carpesium genus that belongs to the Asteraceae family, which is widely distributed in South Korea, China, and Japan
Plantlets grown under R and red and blue (RB) had greater root lengths and number of roots than those grown under W and B
Plantlets grown under RB had a greater shoot diameter, dry matter accumulation, and root system development, which suggested that RB promotes the production of stout plantlets (Figure 2)

Summary

Introduction

Carpesium triste Maxim. is a rare perennial herbal species of the Carpesium genus that belongs to the Asteraceae family, which is widely distributed in South Korea, China, and Japan. Is a rare perennial herbal species of the Carpesium genus that belongs to the Asteraceae family, which is widely distributed in South Korea, China, and Japan. It has a slender stem and is usually 40–100 cm in height. The usage and plant parts extracted for use as herbs in the Carpesium genus vary, according to the oral and literature records in different countries. The shoots of Carpesium abrotanoides L. were used to treat fever and to inhibit inflammation in Korea [3].

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