Abstract
Pollen viability is crucial for wheat breeding programs. The unique potential of the protoplasm of live cells to turn brown due to the synthesis of silver nanoparticles (AgNPs) through rapid photoreduction of Ag+, was exploited for testing wheat pollen viability. Ag+-viability test medium (consisting of 0.5 mM AgNO3 and 300 mM KNO3) incubated with wheat pollen turned brown within 2 min under intense light (~600 μmol photon flux density m-2s-1), but not in dark. The brown medium displayed AgNPs-specific surface plasmon resonance band in its absorption spectrum. Light microscopic studies showed the presence of uniformly stained brown protoplasm in viable pollen incubated with Ag+-viability medium in the presence of light. Investigations with transmission electron microscope coupled with energy dispersive X-ray established the presence of distinct 5-35 nm NPs composed of Ag. Powder X-ray diffraction analysis revealed that AgNPs were crystalline and biphasic composed of Ag0 and Ag2O. Conversely, non-viable pollen and heat-killed pollen did not turn brown on incubation with Ag+-medium in light. We believe that the viable wheat pollen turn brown rapidly by bio-transforming Ag+ to AgNPs through photoreduction. Our findings furnish a novel simplest and rapid method for testing wheat pollen viability.
Highlights
Bread wheat (Triticum aestivum L.), the Nature created allohexaploid, is one amongst the three most widely cultivated staple cereal crops
Pollen viability plays a crucial role in general plant breeding programs, which
Evaluating novel Ag+-medium for testing pollen viability: Within 10–20 min after collection, few pollen grains were incubated in Ag+-viability medium in absence and presence of high-intensity visible light with a photon flux density (PFD) of ~600 μmol m-2s-1, for 1 to 5 min under ambient conditions
Summary
Bread wheat (Triticum aestivum L.), the Nature created allohexaploid, is one amongst the three most widely cultivated staple cereal crops. Wheat pollen turned AgNO3 solution brown within few seconds on incubation in presence of high-intensity light [~600 μmol PFD m-2s-1].
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.