Light microscopic and ultrastructural changes in the ceca of chicks inoculated with human and canine Serpulina pilosicoli.

Abstract

Light microscopic and ultrastructural changes were observed in chicks challenged with North American Serpulina pilosicoli, a weakly beta-hemolytic intestinal spirochete (WBHIS) associated with human and canine intestinal spirochetosis. Chicks in control groups received trypticase soy broth or canine Serpulina innocens. The birds were necropsied at weekly intervals, and the ceca were processed for bacteriologic and pathologic examinations. No WBHIS were isolated from the ceca of chicks in the control groups, but WBHIS with genotypes similar to the parent isolates were isolated from the ceca of chicks inoculated with human and canine S. pilosicoli. Gross examination revealed no significant changes in the ceca of chicks at any time post-inoculation. Light microscopic examination revealed no spirochetal attachment in the ceca of chicks in control groups. In contrast, focal to diffuse thickening of the brush border of the surface epithelium along with dilation of the crypt lumina and mild focal lamina propria heterophil infiltration were present in the ceca of chicks inoculated with human and canine S. pilosicoli. Scanning electron microscopic examination revealed focal to confluent spirochetal attachment mainly in the furrow region at the periphery of the crypt units. Transmission electron microscopic examination revealed spirochetes attached to the brush border of the cecal epithelium, causing effacement of the microvilli and disruption of the terminal web microfilaments. The cecal epithelium of chicks inoculated with the canine S. pilosicoli also had caplike elevations of the apical membrane at the point of attachment of the spirochetes together with large numbers of vesicles in the cytoplasm immediately beneath the terminal web and evidence of spirochetal invasion beyond the mucosal barrier. The changes observed suggested that the mechanism of attachment of human and canine S. pilosicoli to the cecal epithelium of chicks was analogous to but different from that described previously for other attaching and effacing gastroenteric bacterical pathogens of human beings and animals.