Light Induces lnositol Trisphosphate Elevation in Blepharisma japonicum.

Abstract

Abstract- Photoinduced formation of inositol 1,4,5-trisphosphate (Ins[1,4,5]P3 ) was examined using a specific radioimmu-noassay to investigate the molecular mechanisms of light signal transduction mediating photophobic responses in the ciliate Blepharisma japonicum. Application of light stimuli of moderate intensity to dark-adapted cells induced a rapid and significant increase in the basal level of Ins (1,4,5)P3 , with a peak at about 20 s. Thereafter, the level of Ins (1,4,5)P3 declined to the resting value within the subsequent 100 s. Light stimuli of higher intensity raised the cell Ins (1,4,5)P3 content to still higher levels within about 20 s, but the decaying time course was considerably prolonged. In ciliates incubated under dark conditions with agents interfering with the inositol signalling pathway, like neomycin and Li+ the basal levels of Ins (1,4,5)P3 were lower than in control cells. A photoinduced rise of Ins (1,4,5)P3 , content in ciliates treated with neomycin or Li+ was significantly inhibited in a dose-dependent manner. Depolarizing ionic stimuli in dark-adapted ciliates induced no significant alterations of the resting Ins (1,4,5)P3 level, indicating a lack of a contribution of this kind of stimulation to the inositol turnover. These studies are the first in vivo demonstration of a possible role for inositol trisphosphate as a second messenger in the light signal transduction process in the ciliate B. japonicum.