Abstract
Cultivation with 4-8 mumol/l hypericin (specific proteinkinase C inhibitor) activated by light induced high inhibition of the rate of HL-60 cell growth. When hypericin treated cells were not exposed to light growth inhibition was insignificant. Cultivation with light activated hypericin in concentration 16 mumol/l slightly inhibited growth rate of AtT20/D16v-F2 cells. AtT20/D16v-F2 cells did not proliferate in presence of light activated 32 mumol/l hypericin. Evidence of apoptosis was found in HL-60 cells treated with 1-8 mumol/l light activated hypericin, with maximum of apoptotic cells detected after 8 mumol/l light activated hypericin. Light activated hypericin induces both apoptosis and necrosis in dose and time dependent manners in HL-60 cells, but causes only necrosis in AtT20/D16v-F2 cells.
Highlights
Hypericin is a natural pigment, isolated from Hypericum, a genus of plants widely distributed throughout the planet
In our previous studies [11,12,19] we demonstrated apoptosis could be induced in human promyolcyte cell line HL-60 following 6 h incubation with 2% ethanol, 50 nmol/l idarubicin and irradiation by high doses of ionising radiation (20 Gy)
In this study we investigated the ability of protein kinase C inhibitor hypericin to induce apoptosis in HL-60 cell line after photodynamic activation
Summary
Hypericin is a natural pigment, isolated from Hypericum, a genus of plants widely distributed throughout the planet. Hypericin constitutes up to 0,05% of the plant substances. St. John’s Wort, Hypericum perforatum is probably the richest source of hypericin [4]. John’s Wort, Hypericum perforatum is probably the richest source of hypericin [4] Occurring hypericin is often accompanied by pseudohypericin. During the last years some interesting properties of this compounds has been uncovered, which bring them into the consideration as a potential therapeutics in treatment of some types of tumour and viral diseases
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