Light grazing reduces gaseous nitrogen emissions from temperate grassland soils during freeze‒thaw cycles: An intact core incubation study

Abstract

Livestock grazing and soil freeze‒thaw cycles (FTCs) can affect the biogeochemical processes of nitrogen (N) and gaseous N (N2O, NO, and N2) emissions from grassland soils. However, the effect of grazing intensity on soil gaseous N emissions during FTCs and the underlying mechanisms are not clearly understood. In this intact core incubation study, soil gaseous N emissions during two FTCs were simultaneously quantified from temperate grasslands that included grazing exclusion (GE), light grazing (LG), and heavy grazing (HG) in Inner Mongolia. Additionally, the abundance of N cycle-related functional genes and the main soil characteristics were determined to better understand the drivers of gaseous N emissions. The results showed that N2 emissions dominate the gaseous N loss from all investigated soils during FTCs, with cumulative N2 exceeding NO and N2O emissions by factors of 47–135 and 71–161, respectively. Increased soil moisture during thawing promoted N2 and N2O emissions from all three sites, except for N2O at the LG site. However, no obvious NO emission peak was observed from all investigated soils during FTCs. Soil C and N availability and aeration changed by grazing regulated soil N2O fluxes, while the abundances of key functional genes generally did not show significant correlations with gaseous N emissions. Moreover, compared to the GE and HG sites, LG substantially decreased the soil N2O and total gaseous N emissions during FTCs, suggesting that light grazing rather than long-term grazing exclusion could be a promising measure to reduce gaseous N losses during spring thaw. Our results highlighted the importance of the simultaneous determination of all kinds of gaseous N emissions during FTCs for closing the ecosystem N balance and developing appropriate strategies for grassland management.