Abstract
The observation of light emission from bilirubin in an organic solvent as a result of the peroxyoxalate chemiluminescence reaction is reported for the first time. The results presented herein indicate that bilirubin chemiluminescence can be detected in N,N′ dimethylformamide but not in chloroform, dimethylsulfoxide, or pyridine. To elucidate this phenomenon, the effects of solvent on the chemiluminescence intensity were examined as a function of solvent composition. Furthermore, analytical utilization of the present observation for the determination of bilirubin in biological materials was evaluated. Relative and absolute limits of detection were found to be 8.5 nmol 1 −1 and 5.0 ng, respectively, and the response was found to be linear at least over three orders of magnitude. Selectivity for the detection of bilirubin in the presence of a number of common interference species was also examined. Finally, the visible spectrum of bovine serum in the blue-green region was obtained using the present chemiluminescence method and was briefly compared with those obtained from absorption ad fluorescence methods.
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