Light chains of trout myosin isolation and characterization

Abstract

1. 1. Two “alkali” light chains, LC 1 and LC 3, along with one DTNB light chain, LC 2, have been isolated and characterized from the myosin of the white muscle of trout. 2. 2. They are homologous to the corresponding light chains of the carp but LC 1 from trout muscle shows a much lower number of proline residues than LC 1 from carp muscle. 3. 3. The tryptic peptide maps confirm the difference between LC 1 from trout and carp origin. 4. 4. This difference can reflect some differences in the interaction with actin and in the respective enzymic activities of the myosins of the two fish.